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Association of proteins to BMCs is strongly dependent on the targeting peptide. Esterase Est5 (43 kDa) was cloned with or without the PduP‐encapsulation peptide and coexpressed in E. coli BW25113 together with the BMC‐genes encoded by pCC1‐MQ4. Lane 1, Est5 without the encapsulation peptide coexpressed with BMC‐genes; lane 2, Est5 with the PduP‐encapsulation peptide fused to the N‐terminus and coexpressed with BMC‐genes.
