Confirmation of transgenic hairy roots using rol‐B specific primers by PCR reaction. 0.8% agarose gel electrophoresis of PCR‐ amplified products; Lane M: Marker (1 kbp), Lane C+: Agrobacterium A7 Strain as positive control, Lane R, Non‐transgenic Hyoscyamus reticulatus roots as negative control, Lane C−1 and C−2, water as negative control.