Table 1.
Step‐wise analysis of the content of each component during the purification
| Procedure | Crude protein (%) | Nucleic acids (%) | Lipid (%) | Ash (%) | Carbohydrate (%) | Yield (%) |
|---|---|---|---|---|---|---|
| Untreated cells | 45.1 ± 0.24 | 8.9 ± 0.12 | 11.8 ± 0.13 | 7.4 ± 0.41 | 36.8 ± 0.35 | 100 ± 0 |
| Induced autolysis | 26.7 ± 0.076 | 1.4 ± 0.09 | 10.2 ± 0.15 | 6.2 ± 0.12 | 52.7 ± 1.74 | 60.5 ± 0.13 |
| Hot‐water treatment | 28.7 ± 0.18 | 0.3 ± 0.056 | 8.5 ± 0.12 | 3.4 ± 0.14 | 64.5 ± 1.73 | 25.9 ± 0.08 |
| Ultrasonication | 5.3 ± 0.17 | 0.3 ± 0.035 | 5.6 ± 0.08 | 3.9 ± 0.13 | 83.7 ± 1.92 | 17.5 ± 0.05 |
| Isopropanol extraction | 5.0 ± 0.33 | 0.2 ± 0.045 | 0.7 ± 0.05 | 3.3 ± 0.12 | 84.0 ± 2.22 | 15.8 ± 0.11 |
| Protease treatment | 3.3 ± 0.13 | 0.08 ± 0.004 | 0.5 ± 0.03 | 2.7 ± 0.08 | 85.3 ± 2.06 | 11.7 ± 0.04 |
The data were presented as the mean of 3 replicates ± standard deviation.