Table 2.
The genes with significantly downregulated transcription levels of R. toruloides mutants compared with wild‐type studied in this work
| Gene | Fold change of expression levels | Functional description | ||
|---|---|---|---|---|
| Log phase | Stationary phase | Logarithmic phase | ||
| Oxidation‐reduction | ||||
| MNSOD | 20.92 | 40.56 | 40.23 | Oxidoreductase activity |
| CYTB | 12.32 | 32.89 | 38.35 | Respiratory electron transport chain |
| Mo01889 | 4.67 | 30.2 | 13.82 | Oxidoreductase activity, acting on the CH‐CH group of donors |
| ND1 | 12.12 | 27.63 | 10.6 | Oxidoreductase activity |
| HTP6 | 6.07 | 15.61 | 27.6 | Oxidoreductase activity |
| COX1 | 10.36 | 25.49 | 12.8 | Electron carrier activity; oxidoreductase activity |
| MGN | 8.16 | 9.96 | 18.12 | Monooxygenase activity; oxidoreductase activity |
| COX14 | 2.54 | 8.02 | 7.34 | Electron carrier activity; Oxidoreductase activity |
| MGN6 | 3.34 | 11.53 | 14.59 | Oxidoreductase activity |
| RBCS | 3.21 | 19.1 | 10.2 | Ribulose‐bisphosphate carboxylase activity |
| SCD | 2.89 | 15.69 | 9.44 | 3‐oxoacyl‐acyl‐carrier‐protein reductase (NADPH) activity |
| NBP | 8.35 | 13.2 | 18.2 | Oxidoreductase activity |
| Mo01112 | 2.16 | 10.3 | 11.5 | Oxidoreductase activity |
| NBP | 2.98 | 9.19 | 20.9 | Oxidoreductase activity |
| OMO | 3.92 | 8.64 | 6.26 | Oxidoreductase activity |
| HTP15 | 4.37 | 12.86 | 4.31 | O‐methylsterigmatocystin oxidoreductase |
| NAH | 3.54 | 3.15 | 2.77 | Oxidoreductase activity |
| Starch and sucrose metabolism | ||||
| PGM2 | 2.08 | 42.33 | 23.1 | Phosphoglucomutase activity |
| FSG | 1.59 | 13.16 | 16.5 | β‐fructofuranosidase, glycoside hydrolase family |
| TIF2 | 24.70 | 25.2 | 12.57 | Catalytic activity |
| BGS | 5.81 | 11.7 | 16.2 | 1,3‐beta‐glucan synthase |
| ACT | 2.16 | 17.9 | 14.47 | Amino acid transmembrane transporter activity |
| CRP | 10.43 | 12.3 | 20.2 | Inorganic cation transmembrane transporter activity |
| CAT | 3.17 | 5.27 | 8.4 | Lactate transmembrane transporter activity |
| HTP4 | 7.44 | 8.53 | 3.26 | Actin cytoskeleton organization |
| Amino acid metabolism | ||||
| HisF | 2.39 | 4.34 | 19.5 | Glutamine amidotransferase |
| PCI4 | 5.22 | 20.02 | 16.2 | Carboxamide isomerase |
| PPAP41 | 3.4 | 12.23 | 17 | Phosphoribosyl‐ATP pyrophosphohydrolase |
| ASL | 1.71 | 4.69 | 9.56 | Argininosuccinate lyase |
| GDD1 | 2.65 | 8.85 | 6.13 | Glutamate dehydrogenase |
| SAFC2 | 6.63 | 7.24 | 3.9 | Aspartate aminotransferase, cytoplasmic |
| PIPA | 4.67 | 3.03 | 5.2 | Proline iminopeptidase |
| DAT | 2.13 | 3.74 | 4.85 | Diamine N‐acetyltransferase |
| TAF1 | 2.4 | 4.98 | 2.33 | Tyrosine aminotransferase |
| Oxidative phosphorylation | ||||
| Mo06061 | 4.78 | 20.4 | 12.3 | Establishment of mitochondrion localization |
| RTG2 | 2.12 | 18.5 | 11.2 | Protein phosphorylation |
| ATPI | 5.26 | 14.9 | 9.39 | ATP synthesis coupled proton transport |
| ATP9 | 4.32 | 12.5 | 5.86 | Hydrogen ion transmembrane transporter activity |
| ATPE | 12.41 | 8.33 | 6.12 | ATP synthesis coupled proton transport |
| STA | 6.76 | 6.2 | 3.13 | Ras GTPase activator activity |
| Protein binding and processing | ||||
| PPG3 | 4.39 | 28.23 | 22.3 | Protein binding |
| ALG | 7.75 | 25.5 | 21.54 | Calnexin, protein processing |
| GFA | 2.51 | 14.1 | 13.78 | Formaldehyde‐activating enzyme |
| SUR7 | 2.2 | 6.85 | 11.52 | Protein binding; |
| RNA binding & transcription | ||||
| PPG4 | 5.89 | 20.33 | 12.09 | mRNA processing |
| RPS7 | 6.5 | 17.56 | 7.4 | RNA binding |
| GRP | 9.45 | 11.9 | 9.22 | Nucleic acid binding |
| RPL2 | 1.34 | 8.83 | 4.52 | RNA binding |
| PMAR | 10.2 | 5.98 | 7.06 | RNA binding |
| PPG47 | 9.11 | 3.77 | 4.54 | C2H2 zinc finger domain binding |