Figure 4.

Modification of CtAAAH via protein engineering: (A) in vivo activity of wild‐type (wt) CtAAAH and its mutants, dark gray and blue bars indicates the activities toward phenylalanine and tryptophan, respectively. (B) Cell growth (dark gray) and 5HTP production (blue) during fermentation by fed‐batch using shaken flasks. BL21(DE3)∆tnaA (squares), BL21(DE3) pCtAAAH‐W192F (diamonds), BL21(DE3) cofactor (triangles), BL21(DE3) cofactor pCtAAAH‐W192F (circles). Bars present in the graphs correspond to the standards errors means (SEM) from three independent experiments.