Table 1.
Bacterial strains and plasmids used in this study
| Characteristics | Source | |
|---|---|---|
| Strains | ||
| C. taiwanensis | DSM No.: 17343, type strain | DMSZ |
| E. coli BL21(DE3) | fhuA2 [lon] ompT gal (λDE3) [dcm] ∆hsdS λDE3 = λsBamHIo ∆EcoRI‐B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5 | NEB |
| E. coli BL21(DE3)∆tnaA | BL21(DE3)∆tnaA | This study |
| E. coli BL21 cofactor | BL21(DE3)∆tnaA pBbE1k‐2 | This study |
| Plasmids | ||
| pACYCDuet | Novagen | |
| pBbE1k‐2 | pBbE1K inserted with DHPR, PCD | 32 |
| pCtAAAH | pACYCDuet inserted with C. taiwanensis AAAH | This study |
| pCtAAAH L113Y | pACYCDuet inserted with C. taiwanensis AAAHL113Y | This study |
| pCtAAAH W192F | pACYCDuet inserted with C. taiwanensis AAAHW192F | This study |
| pCtAAAH Y222H | pACYCDuet inserted with C. taiwanensis AAAHY222H | This study |
| pCtAAAH S223C | pACYCDuet inserted with C. taiwanensis AAAHS223C | This study |
| pCtAAAH P229A | pACYCDuet inserted with C. taiwanensis AAAHP229C | This study |
| pCtAAAH Y244C | pACYCDuet inserted with C. taiwanensis AAAHY244C | This study |