Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used in cloning
| Primer | Sequence (5′‐3′) | Template | RE* |
|---|---|---|---|
| P1 | ATGGGCGATCGGAAAACCAAA | SaFPPS F | ‐ |
| P2 | CTTCTGCCGCTTGTATATCTTCGC | SaFPPS R | ‐ |
| P3 | GAGACCATGGGCGATCGGAAAACC | SaFPPS F | Nco I |
| P4 | AAGGATCC GCCGCTGCCCTTCTGCCGCTTGTATA | SaFPPSLnk R | Bam HI |
| P5 | AGCCTGATTGTTGAAGATGTTATTCGTCCG | AaECS F | ‐ |
| P6 | TTAGGTGATGATGGCATCCACAAACAG | AaECS R | ‐ |
| P7 | ACGGGATCCAGCCTGATTGTTGAAGATGTTA | AaECS F | Bam HI |
| P8 | AAGCGGCCGCTTAGGTGATGATGGCATC | AaECS R | Not I |
Restrictions sites are underlined and linker sequences are in bold.
