Figure 2.

Online data of a typical cultivation in a disposable bag reactor. The dissolved oxygen level (line: dash dot dot) was controlled by the rocking motion (line: dash). After 4.5 h, when the rocking capacity of the system reached its maximum (42 Rpm), oxygen supply (line: solid) started. The oxygen supply increased during the cultivation, because of the cell activity. During the whole process no oxygen limitation was observed and the internal pressure (line: dot) was below the maximal pressure of 50 mbar.