Figure 3.
Offline data of a typical cultivation in a disposable bag reactor. After approximately 4 h of lag phase, the bacteria entered the exponential growth phase, noticeable by the OD600 (symbol: square, line: short dot) and the CDW (symbol: circle, line: dash dot). The exponential growth phase took place between 4 and 8 h of cultivation time. Connected to the bacteria concentration, the protein concentration (symbol: triangle, line: dot) and the polySia concentration (symbol: triangle, line: short dash dot) increased. The glucose (symbol: square, line: dash) was consumed after 10.5 h. At the end of the cultivation CDW reached 7.1 g L−1, the protein concentration 160 mg L−1 and the polySia concentration 243 mg L−1.