Table 1.
Experimental factors and levels used for the investigation of SCMA for purifying cell culture‐derived influenza virus A/Puerto Rico/8/34 using a DoE approach
| Factor | Abbreviation | Level | ||
|---|---|---|---|---|
| Low | Center | High | ||
| Ligand density (μmol cm−1) | LD | 14 | 19.5 | 25 |
| Membrane permeability (mL (min bar cm2)−1) | Lp | 75 | 97.5 | 120 |
| Flow rate (mL min−1) | Q | 0.112 | 0.336 | 0.560 |
| [NaCl] in the step elution (mM) | [NaCl] | 200 | 1100 | 2000 |
| Conductivity of the feed stream (mS cm−1) | Feedcond | 4.0 | 6.5 | 9.0 |
| Virus titer in the feed stream (logHAU) | FeedHAU | 3.0 | 3.7 | 4.0 |