Table 4.
Factors and responses for the robust set point predicted by Monte Carlo simulation (resolution 32, 10 000 simulations per point, 95% confidence level. The responses obtained confirm the validity of the selected model. Responses as mean ± standard deviation of 3 technical replicates
| Factor | Predicted | Implemented |
|---|---|---|
| LD (μmol cm−2) | 20.6 ± 1.5 | 20.0 |
| Lp (mL (min.bar.cm2) −1) | ‐ | 106 |
| Q (mL min−1) | 0.375 ± 0.060 | 0.380 |
| [NaCl] (mM) | 560 ± 197 | 560 |
| Feedcond (mS cm−1) | 4.67 ± 0.43 | 4.7 |
| FeedHAU (logHAU) | 3.95 ± 0.1 | 3.8 ± 0.01 |
| Response | Predicted | Obtained |
|---|---|---|
| FTvirus (%) | 2.6 ± 0.8 | 2.1 ± 1.5 |
| Yield (%) | 58.2 ± 3.3 | 57.4 ± 0.6 |
| DNAprod (pgDNA HAU−1) | 5.9 ± 0.8 | 5.1 ± 0.2 |
| Protprod (ngprot HAU−1) | 1.4 ± 0.1 | 1.2 ± 0.02 |