Figure 6.

Fluorescent in situ hybridization (FISH) analysis of Kcnmb4 mRNA and its overlapping Kcnmb4os1 AS lncRNA in mouse testis. (A) Diagram showing the structure and orientations of Kcnmb4 and Kcnmb4os1 transcripts. White rectangles represent non-coding exons. For the sense transcript, only the last coding exon is shown (coding region in black, 3ʹ UTR in white). (B) Co-localization of the sense and AS transcripts in the chromatoid body of RS. Red: AS lncRNA; green: mRNA. The right frame shows co-staining with both probes. (C) Co-immunostaining with anti-MVH antibody (green) in RS as a marker for the chromatoid body. The AS is shown in red, while the mRNA is shown in orange in this case. The image to the right shows the triple staining. All the sections were co-stained with DAPI. Bars: 10 µm.