Figure 1.

Cnot1-KO MEFs undergo cell death. (A) Lysates were prepared from Cnot1-flox MEFs that were infected with mock or Cre-expressing retrovirus and subjected to immunoprecipitation with anti-CNOT3 antibody. CNOT3 are shown in red to indicate a precipitated molecule. Lysates and immunoprecipitates (IP) were analysed by immunoblot with the indicated antibodies. (B) Quantification of the immunoblot data in Fig. 1A. Relative band intensities normalized to those of IP or lysates in control MEFs are shown (n = 3). Values represent means ± standard error of the mean (S.E.M.). (C) Morphology of Cnot1-flox MEFs infected with mock (Control) or Cre-expressing retrovirus (Cnot1-KO). Photographs are at the same magnification and represent one of the three independent experiments. Dead cells that were about to lose adhesion were observed in Cnot1-KO MEFs. (D) Cell death was assessed by propidium iodide uptake using flow cytometry (n = 3). Values in the graphs represent mean ± S.E.M. *P< 0.05, **P< 0.01, ***P< 0.001.