Figure 2.

Suppression of CNOT7/8, but not CNOT6/6L, affects viability of MEFs. (A) Morphology of MEFs with the indicated genotypes shown as in Fig. 1B. Mock and Cre represent cells infected with retrovirus. (B) Cell death was assessed as in Fig. 1D (n = 3). Values represent means ± S.E.M. ***P< 0.001. (C) Lysates were prepared from Cnot8-flox MEFs, which were infected with mock or Cre-expressing retrovirus and subjected to immunoprecipitation with anti-CNOT3 antibody. CNOT3 is shown in red to indicate a precipitated molecule. Lysates and IP were analysed by immunoblot with the indicated antibodies. WT: wild-type, KO: knockout. (D) Lysates were prepared from MEFs with the indicated genotypes and subjected to immunoprecipitation with anti-CNOT3 antibody. Lysates and IP were analysed by immunoblot.