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. 2020 Jan 10;17(3):403–416. doi: 10.1080/15476286.2019.1709747

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 6. — Foxo3 and Txnip mRNAs are upregulated and stabilized in Cnot1-KO and Cnot7/8-dKO MEFs. (A) qPCR analysis of the indicated mRNAs in Cnot1-KO, Cnot7/8-dKO and Cnot6/6l-dKO MEFs together with their controls, as in Fig. 5. Relative mRNA levels were determined by qPCR and normalized to the Gapdh mRNA level (n = 3). (B) Decay curves of mRNAs. Total RNAs were prepared from the indicated MEFs treated with Act. D (0, 4 or 8 h). Relative mRNA levels were determined as in (A). mRNA levels without Act. D treatment (0 h) were set to 100% (n = 3). All values represent means ± S.E.M. *P< 0.05, **P< 0.01, ***P< 0.001.