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. 2019 Jun 23;16(3):548–561. doi: 10.1080/15548627.2019.1632104

Figure 7.

Figure 7.

GlcN enhances IAV, EV71, and VSV replication in vitro. (A–C) MDCK, RD, and Huh7 cells were treated with 10, 1, or 1 mM GlcN and harvested at 24 h after treatment. The levels of LC3 and SQSTM1 were detected by western blotting, using ACTB as a loading control. LC3-II:ACTB ratios were quantified by densitometry. (D) MDCK cells were infected with IAV (H3N2; MOI = 0.1) and treated with 10 mM GlcN for 24 h. The mRNA levels of the IAV NP gene were quantified by RT-qPCR. (E) RD cells were infected with EV71 (MOI = 1) and treated with 1 mM GlcN for 12 h before harvesting the cells. EV71 RNA levels were determined by RT-qPCR with EV71 VP1-specific primers. (F) Huh7 cells were infected with VSV (MOI = 1) and treated with 1 mM GlcN for 24 h. The culture medium was harvested, and the viral loads were determined by RT-qPCR with VSV-specific primers. *P < 0.05; **P < 0.01; ns, not significant. IAV, influenza A virus; EV71, enterovirus 71; VSV, vesicular stomatitis virus.