Fig. 2.

MFG-E8 deficiency enhances the inflammatory response in vitro. Bone marrow stromal cells, isolated from wild-type (WT) and MFG-E8 knock-out (MFG-E8 KO) mice were differentiated for 7 days into osteoblasts. Cells were treated with LPS (1 μg/mL) or vehicle (CO) for 48 hours. (A) Gene expression of interleukin-6 (IL-6) and (B) tumor necrosis factor-a (TNF-α) was determined using qPCR and normalized to β-actin. n = 4–5. *p < 0.05;**p < 0.01; ***p < 0.001.