Fig. 5. FTO induces phosphorylation of AMPK in C3H10T1/2 cells.

(A) C3H10T1/2 cells were transfected with pCMV-FTO (2 μg) for 6 h and then incubated for a further 12 h. Western blot assay analysis was performed using the indicated antibodies. (B) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) for 12 h. Western blot analysis was performed using the indicated antibodies. All experiments were independently repeated at least three times.