Skip to main content
. 2020 Jan 14;43(1):58–65. doi: 10.14348/molcells.2019.0136

Fig. 6. FTO and p-AMPK induce mild ER stress.

Fig. 6

(A) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) and Com.C (1 μM) for 1 day. RT-PCR was performed using total RNA isolated from cells. (B and D) C3H10T1/2 cells were transfected with pCMV-FTO (2 μg) for 6 h and then treated with Com.C (1 μM) for 24 h. (C and E) C3H10T1/2 cells were transfected with pc-DNA3.0-AMPK (2 μg) and siFTO for 6 h and then incubated for a further 12 h. RT-PCR (B and C) and western blot (D and E) analyses of ER stress markers (ATF4 and CHOP) were performed. All experiments were independently repeated at least three times.