Figure 5.

Triple therapy with Tim-3, PD-1 and Lag-3 mAbs synergistically increased the antitumor activity.

a-b. MC38 tumor-bearing mice were treated with Tim-3, PD-1, Tim-3/PD-1 mAbs or IgG control, 24 h later, tumor-infiltrating lymphocytes were analyzed for Lag3 expression by flow cytometry. (a). Representative flow plots of Lag3 expression on tumor-infiltrating CD4⁺ and CD8 T⁺ cells. (b). Statistical analysis of Lag3 expression depicted in A (n = 4). (c). Mean tumor volumes of MC38 tumor-bearing mice treated with indicated mAbs (n = 5). Data are presented as mean ± SEM, *P < .05, **P < .005, one-way ANOVA test and Student’s t test were performed.