Fig 4. Haematoxylin/eosin (H&E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 PCR for MKPV DNA using DNA extracted from FFPE kidney shavings of necropsies from the same sites.

For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H&E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1^–/–mice (+ve) or MKPV-free Rag1^–/–mice (-ve, sourced from Australian BioResources, Mittagong NSW).