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. 2019 Dec 26;19(2):375–389. doi: 10.1074/mcp.RA119.001779

Fig. 4.

Fig. 4.

CD73 is up-regulated in the radioresistant cells. A, A representative mass spectrum (MS1 spectrum) of a lysine-containing peptide (GPLASQISGLYLPYK) derived from protein CD73. B, qRT-PCR analysis of CD73 mRNA in the indicated cells. Values are the mean ± S.E. of three separate sample preparations. *p, < 0.05. C, Western blot analysis of CD73 protein expression in the indicated cells. P, the parental cells; #1–8, different clones of the radioresistant cells (clone #6 was MIA PaCa-2-R). Actin was used as a loading control.