CD73 enhances radioresistance.
A, Western blot analysis of CD73 expression in vector-transformed stable cells (Vector) and the stable cells that overexpressed CD73 in MIA PaCa-2 cells (CD73over). B, Clonogenic cell survival assay of the indicated cells. C, Western blot analysis of CD73 expression in MIA PaCa-2-R cells and the cells in which CD73 was knocked down in MIA PaCa-2-R cells (shCD73). D, Clonogenic cell survival assay of control cells (shGFP) and the shCD73 cells. Actin and tubulin were used as loading controls. Values in B and D are the mean ± S.E. of three separate sample preparations. *p, < 0.05; **p, < 0.01; ***p, < 0.001.