Fig. 3.

The CD16a N162-glycan is predominantly a monosialylated biantennary complex-type with some hybrid/oligomannose. The relative abundance represents the averages of each species from datasets originating from individual donors and donor pools and the pie charts represent the average abundance of each N-glycan type. A, Relative abundance of F158 glycopeptides (1 and 7). B, Relative abundance of glycopeptides for a single V158-specific peptide. C, A representative MS2 spectrum for most abundant N162 F158 glycopeptide. D, A representative MS2 spectrum for most abundant N162 V158 glycopeptide. Cartoons indicate one possible glycan configuration consistent with the composition, isobaric ions are not distinguished.