Fig. 6.

Purified CD32a contains predominantly complex-type biantennary N-glycans. A, CD32a contains 2 N-glycosylation sites on the extracellular domain. These sites are modeled from the most abundant forms found on monocytes; the ribbon diagram and surface rendering are based on known structures of CD32a determined by X-ray crystallography. The most abundant N-glycans at N64 (B) and N145 (C). D, ESI-MS/MS identified peptides and glycopeptides covering 61% of the CD32a sequence. Glycosylated Asn residues are shown in red. Representative MS2 spectra for the most abundant N-glycoforms at N64 (D) and N145 (E). Data in B, C, E and F are from the MoB pool.