Table III. Equilibrium dissociation constants (KD) from SPR analysis of the CD16a and IgG1 Fc interaction (Errors represent errors of fit to the Langmuir binding isotherm; this experiment is representative of three independent experiments).
| IgG1 Fc G0F | IgG1 Fc G0 | |||
|---|---|---|---|---|
| Receptor | KD (nM) | error | KD (nM) | error |
| CD16a V158-CT | 500 | 30 | 100 | 10 |
| CD16a V158-Man9 | 170 | 20 | 40 | 10 |
| CD16a V158-Hybrid | 220 | 20 | 70 | 10 |
| CD16a F158-CT | 1500 | 100 | 240 | 40 |
| CD16a F158-Man9 | 1300 | 200 | 200 | 70 |
| CD16a F158-Hybrid | 1000 | 100 | 200 | 10 |