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Autophagy induction in TNBC cells. A, Western blot analyses to evaluate the protein expression levels of known autophagy markers and rpS6 phosphorylation in DMSO, EGFRi, ROCKi and EGFRi+ROCKi treated MDA-MB-231 cells. Hsp90 is used as a loading control. B, Western blot analyses of LC3-II (MAP1LC3-II) and phosphorylated rpS6 levels in Hs578T, Cal120 and HCC1806 cells. Actin is used as a loading control.
