Figure 4.

An unannotated exon in the regulator of emerin Lmo7 that is specific to heart and skeletal muscle. (a) Genome browser tracks show RNA-Seq read coverage over Lmo7, with the novel exon highlighted by a red arrowhead. GSE4 and GSE5 refer to two rat body map datasets produced by independent labs. E(PSI) values represent expected percent spliced in (PSI) values calculated by MAJIQ. (b) Again showing genome browser tracks, but for mouse C2C12 myogenesis data where each replicate is a representative sample from data from an independent lab. (c) PhyloP conservation track shows that the genomic region containing the novel exon is highly conserved between vertebrates. (d) Alignment of the mouse and rat exon amino acid sequences shows the high level of conservation, with non-conserved amino acid residues highlighted in red. Blue and orange lines indicate regions of the sequence where serine phosphorylation motifs and serine binding motifs respectively were identified by PhosphoMotif Finder.