Table 2.
Fetuses exposed to ZIKV/control media at 25 days of development and sampled at 110 days of development.
| ZIKV RNA U/g or ml |
ZIKV log10 TCID50/ml |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Litter | Fetus | Fetal appearance | Cranium diameter, mm | Cranium length, mm | Cortisol in AF, ng/ml |
Placenta | AM | AF | Plasma | Brain | AF | ZIKV IgGc Ab log2 in plasma |
| G30 Controla | ||||||||||||
| 1M (IA) | - | 56 | 130 | 10.52 | - | - | - | - | - | - | - | |
| 2M (IA) | - | 55 | 125 | 18.99 | - | - | - | - | - | - | - | |
| 3F (IA) | - | 57 | 125 | 9.54 | - | - | - | - | - | - | - | |
| 4M (IA) | - | 56 | 115 | 15.09 | - | - | - | - | - | - | - | |
| 5F | - | 52 | 140 | 38.43 | - | - | - | - | - | - | - | |
| 6M | - | 50 | 130 | 13.45 | - | - | - | - | - | - | - | |
| 7F | - | 52 | 130 | 97.86 | - | - | - | - | - | - | - | |
| G27 ZIKV+b | ||||||||||||
| 1na (IA) | resorption | na | na | na | na | na | na | na | na | na | na | |
| 2M (IA) | dead fetus | 51 | 135 | na | 1.6 | 3.9 | 2.3 | - | - | - | 6 | |
| 3na (IA) | resorption | na | na | 121.12 | 3.2 | 1.4 | 2.3 | na | na | na | 3d | |
| 4na (IA) | resorption | na | na | na | 2.3 | 1.5 | na | na | na | na | na | |
| 5M | meconium staining | 50 | 120 | 74.05 | - | 1.2 | < 0.1 | - | - | - | - | |
| 6F | - | 52 | 125 | 34.26 | - | - | - | - | - | - | - | |
| 7F | - | 49 | 125 | 46.03 | - | - | - | - | - | - | - | |
| 8M | meconium staining | 43 | 115 | 39.07 | - | - | - | - | - | - | - | |
| 9F | - | 50 | 125 | 60.83 | - | - | - | - | - | - | - | |
| 10M | - | 54 | 122 | 26.79 | - | - | - | - | - | - | na | |
| 11M | - | 50 | 120 | 106.23 | - | - | - | - | - | - | - | |
| 12F | meconium staining | 51 | 122 | 61.83 | - | - | - | - | - | - | - | |
aG30 Control – a control gilt; fetuses #1–4 were inoculated with virus-free media into the allantoic cavity (IA).
bG27 ZIKV+ – fetuses #1–4 were inoculated with ZIKV IA. Relative log10 TCID50 values were defined as RNA units (U) and expressed as ZIKV RNA U per gram (g) of tissues or milliliter (ml) of fluids [8].
cIgM antibodies (Ab) were below the detection limit. M/F – Male/Female. AM – amniotic membrane. AF – amniotic fluid. Brain – PCR data for the cerebrum and cerebellum. “-” – below the detection limit. na – not available.
dAb were tested in AF.