Figure 4.

TGEV Nsp2 activated NF-κB.

(a) ST cells were co-transfected with pNF-κB-Luc, pRL-TK, and the indicated expression plasmid encoding the TGEV protein, or truncated segments. At 36 h post-transfection, cells were harvested and analyzed by western blotting using the anti-HA antibody and cell extracts were prepared for luciferase reporter gene assays. P values < 0.05 (*) and < 0.01 (**) were considered to be statistically significant and highly significant, respectively.(b) Increasing quantities of Nsp2 expression plasmids (0 μg, 0.5 μg, 1 μg, and 1.5 μg) were co-transfected with pNF-κB-Luc and pRL-TK into ST cells. Cells were harvested and analyzed by western blotting using the anti-HA antibody and luciferase activity measurement at 36 h post-transfection. Results are representative of three independent experiments. Data are presented as mean ± SD values. P values < 0.05 (*) and < 0.01 (**) were considered statistically significant and highly significant, respectively.