Figure 7.

The amino acids 1–120 of Nsp2 were responsible for the activation of the NF-κB signaling pathway.

(a) ST cells and (b) IPEC-J2 cells were co-transfected with pNF-κB-Luc, pRL-TK, and the distinct truncated mutants of Nsp2 expression plasmids (schematic diagrams of the structure of the TGEV-truncated Nsp2 mutants are on the left of figure a and b). The empty pCMV-HA vector was used as a control. Cells were harvested and analyzed for luciferase activity at 36 h post-transfection. Results are representative of three independent experiments. Data are presented as mean ± SD values. P values < 0.05 (*) and < 0.01 (**) were considered statistically significant and highly significant, respectively.