Table 3.
Predicted pH where binding is mainly expected based on presence of the BabA, SabA and LabA adhesins in the genome of the H. pylori strains (Table 1) and glycans known to confer binding identified on the mucins (Table 2).
|
H. pylori |
Mucintype and mucin glycans |
|||||
|---|---|---|---|---|---|---|
| Strain | Patient | Human gastrictumor | Human gastric normal | Monkeygastric normal | Human gastric normal 3IS | Human gastric normal 4ID |
| ID | outcome | (Leb, SLea/x, S) | (Leb, LacdiNAc) | (SLea/x) | (Leb, LacdiNAc) | (SLea/x, LacdiNAc) |
| 771/99 | PUD | pH 2 and 7 | pH 7 | pH 7 | pH 7 | pH 7 |
| 417/02 | PUD | pH 2 | - | - | - | - |
| 441/02 | PUD | pH 2 and 7 | pH 7 | pH 7 | pH 7 | pH 7 |
| 559/02 | PUD | pH 2 and 7 | pH 7 | - | pH 7 | pH 7 |
| 1776/05 | PUD | pH 2 and 7 | pH 7 | pH 7 | pH 7 | pH 7 |
| 1089/03 | PUD | pH 2 | - | - | - | - |
| 1152/02 | PUD | pH 2 and 7 | pH 7 | - | pH 7 | - |
| 1846/05 | PUD | pH 2 and 7 | pH 7 | pH 7 | pH 7 | pH 7 |
| 1790/05 | PUD | pH 2 and 7 | pH 7 | pH 7 | pH 7 | pH 7 |
| 1198/04 | PUD | pH 2 | pH 7 | - | pH 7 | pH 7 |
| 499/02 | PUD | pH 2 and 7 | pH 7 | - | pH 7 | pH 7 |
| 517/99 | NUD | pH 2 | pH 7 | - | pH 7 | pH 7 |
| 892/99 | NUD | pH 2 | pH 7 | - | pH 7 | pH 7 |
| 207/99 | NUD | pH 2 | pH 7 | - | pH 7 | pH 7 |
| 173/00 | NUD | pH 2 and 7 | pH 7 | pH 7 | pH 7 | pH 7 |
| 655/99 | NUD | pH 2 and 7 | pH 7 | pH 7 | pH 7 | pH 7 |
| 1786/05 | NUD | pH 2 and 7 | pH 7 | pH 7 | pH 7 | pH 7 |
| 36/00 | NUD | pH 2 and 7 | pH 7 | pH 7 | pH 7 | pH 7 |
| 565/99 | NUD | pH 2 and 7 | pH 7 | pH 7 | pH 7 | pH 7 |
| 1500 | NUD | pH 2 and 7 | pH 7 | - | pH 7 | pH 7 |
Only pH 2 and 7 were included since the pH dependency slope for the binding modes can vary between strains. Although the monkey gastric normal sample is negative for Leb, it carries other fucosylated structures that have potential for interacting with BabA. However, in the table we only included Leb as BabA ligand as this is expected to be the main structure conferring binding. Similarly, only the human gastric tumor sample is listed for expected theoretical interaction with H. pylori at pH 2, as this sample carries sulfated structures in addition to sialylated ones, whereas the other samples carry sialylated structures whereof only 11% are expected to remain negatively charged at pH2. S, Sulfate; -, absence of known ligand-adhesion interactions; NUD, non-ulcer dyspepsia; PUD, peptic ulcer disease.