Figure 2.

FN enhanced IL-6 and TNFα responses of macrophages to PAMPs. Peritoneal macrophages (2x10⁶ cells/well) from C57L/B6 mice were cultured in 24 well culture plate, and the cells were stimulated with LPS, PGN, LTA, Man and Zym for four h. The supernatants were collected and assayed for TNFα (a) and IL-6 (b) production with ELISA kit. Data are means of triplicates. (n = 3, *p < 0.05). Statistical comparisons among treatment groups were performed by randomized-design two-way ANOVA, followed by unpaired Student’s t-test for two groups using Prism software (Graph Pad Inc., La Jolla, CA). Statistical significance was defined as a P value of less than 0.05.