Table 2.
Real Time PCR Primers used in this study.
| Function | Genes | Gene product | Oligonucleotide sequence (5’ to 3’) | product size (bp) |
|---|---|---|---|---|
| AI-2 synthesis, biofilm formation, cell motility and exopolysaccharide formation | luxS | S-ribosylhomocysteinase | F: ACGAGTGCATCTGGTAAGTG R: CAATGGAAGACGTGCTGAAAG |
87 |
| pfs | 5’-methylthioadenosine/S-adenosylhomocysteine nucleosidase | F: CGGCAGAACCGGTGTTAATAAT R: TGAAATCGGGCATCGGTAAAG |
96 | |
| hha | Hemolysin expression-modulating protein | F: GTGATCTGCGGCTGAGTAAA R: ACGTCGTTGCCAGACAAT |
103 | |
| wzb | Protein-tyrosine-phosphatase | F: TCGTTATCCCAGTGACCAAAC R: GTGTCGCAACTATGACCTGAT |
114 | |
| ompG | Outer membrane protein G precursor | F: CGGTTGGCTGTCGATGTATAA R: AGGTATATTGCAGACCCGTTTC |
95 | |
| rcsB | Transcriptional regulator RcsB | F: CAAGTACATCAAGCGCCATTTC R: CCCTTCGATATCCAGATCCAATAC |
103 | |
| motB | Flagellar motor protein B | F: AGGCTAATACGGTTGGGAATAC R: AGAATCGCCCGATGTTTAGAA |
109 | |
| flgN | Flagella synthesis chaperone protein FlgN | F: CCAGTAACCAGCCGTTATGTT R: TACGCAGGAAAGAACCCAATAC |
117 | |
| fliP | Flagellar biosynthetic protein FliP | F: AGCCATTCAGCGAAGAGAAA R: GTCTGGCAAACAACCCTAAATC |
117 | |
| cheW | Purine-binding chemotaxis protein CheW | F: CATCCACCTGGCTGAACTTA R: GTAACACGGATTGCGAACAC |
106 | |
| nlpC | Probable lipoprotein NlpC precursor | F: GCATCGTCACAACCACAAATC R: GGTTTGAACGACCAGCTACA |
103 | |
| Virulence- associated genes | ompA | Membrane protein | F: TGACCGAAACGGTAGGAAAC R: GGAATACCAGTGGACCAACAA |
99 |
| fimC | Fimbrial chaperone protein | F: GCTGGCAGGTATCCTGATATTC R: TGCCCTGCCGGATAAATTAC |
99 | |
| IucD | IucD protein | F: GTCCGGAGAAGCCTGAAATA R: GAGAAGCGGCGGAAATAAAC |
114 | |
| fyuA | Ferric yersiniabactin uptake A | F: CTTCCCTTCCGGTTCGTTAATC R: GGTACAGCCCAAACACCATATC |
119 | |
| iss | Serum survival protein | F: CGCTCTGGCAATGCTTATTAC R: GAAATGATGGGTGATGGTTTCC |
100 | |
| vat | Vacuolating autotransporter toxin | F: CTGAACCGCGTCCAGATTAT R: ACTCCACGGCAGGAAATATG |
104 | |
| Cell division, DNA processing, and morphology | bolA | BolA protein | F: CAGTACTTTAGCGGAGGAACTC R: CAACCCTTCCCACTCCTTAAT |
82 |
| mreD | Rod shape-determining protein MreD | F: CCGGTCTGAAAGAGACGTTAAT R: TTCCGCAACCTCGCATTAT |
115 | |
| csrA | Carbon storage regulator A | F: GTAACTGGACTGCTGGGATTT R: CCAGGTACGTATTGGCGTAAA |
100 | |
| murD | UDP-N-acetylmuramoylalanine- D-glutamate ligase | F: GCGTGGTTAATGCTGATGATG R: CCTGCTGATGATTCAGGTGATA |
108 | |
| lpp | Major outer membrane lipoprotein precursor | F: CGGTAATCCTGGGTTCTACTCT R: TGCTCAGCTGGTCAACTTTAG |
108 | |
| Small molecules metabolism | frwC | Fructose-like-2 IIC component | F: AGCAGGGCAGCATTGTTAT R: GCAAATCAGCATGAAGGCATAG |
104 |
| Fpr | Ferredoxin-NADP reductase | F: GTTCCTGCATCAGAGGTAGATAG R: TACAGCGATTGGCCCTTATT |
128 | |
| mtlR | Mannitol operon repressor | F: CGTCATTAACCGCCAGGAATA R: GTTCACCAAAGGGTCCAAGTA |
122 | |
| ugpC | SN-glycerol-3-phosphate transport ATP-binding protein UgpC | F: CTGATCGTGGGTAACGTAGAG R: GCAGTGTTCCTGTTTGATGAG |
126 | |
| thiH | Thiazole biosynthesis protein ThiH | F: GCATAAGTCGCCTCGTGATATG R: GACGGAATACGCCGAGTTAAAG |
81 | |
| Housekeeping gene | GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | F: CGGTACCGTTGAAGTGAAAGA R: ACTTCGTCCCATTTCAGGTTAG |
99 |