Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Danio rerio) | Tupfel Long Fin (TL) | ZIRC | ZDB-GENO-990623–2 | |
| Genetic reagent (Danio rerio) | Tg(myl7:dsRED)s879Tg | Chi et al., 2008 | ZDB-FISH-150901–3078 | |
| Genetic reagent (Danio rerio) | Tg(myl7:GFP)twu34Tg | Huang et al., 2003 | ZDB-FISH-150901–212 | |
| Genetic reagent (Danio rerio) | Tg(phd3:GFP)sh144 | Santhakumar et al., 2012 | ZDB-FISH-150901–26851 | |
| Genetic reagent (Danio rerio) | Tg(gata4:EGFP)ae1 | Heicklen-Klein and Evans, 2004 | ZDB-FISH-150901–14762 | |
| Genetic reagent (Danio rerio) | TgBAC(nppa:mCitrine) | This paper | More info on generation of this line can be obtained from the Materials and methods section ‘Transgenic zebrafish lines and cryoinjury’. | |
| Cell line (Mus musculus) | TetRE-caErbb2 X MYH6-tTA X MYH6-cre ROSA26-tdTomato | D'Uva et al., 2015 | More info on how to obtain primary cell line can be obtained from Materials and methods sections ‘Transgenic mouse lines and animal procedures’ and ‘Pharmacological inhibition of glycolysis’. | |
| Biological sample (Mus musculus) | TetRE-caErbb2 X MYH6-tTA hearts |
D'Uva et al., 2015 from: Xie et al., 1999 and Yu et al., 1996. |
The Jackson Laboratory, stock no. 010577; | |
| Biological sample (Danio rerio) | Tg(cmlc2:CreER)pd10 x Tg(β-act2:BSNrg1)pd107hearts | Gemberling et al., 2015 | ZDB-FISH-150901–25249 x ZDB-FISH-150901–25354 |
|
| Biological sample (Danio rerio) | Tg(gata4:EGFP)ae1hearts | Lin et al. 2009 | ZDB-FISH-150901–14762 | |
| Biological sample (Danio rerio) | Tg(phd3:GFP)sh144hearts | Santhakumar et al., 2012 | ZDB-FISH-150901–26851 | |
| Antibody | Anti-AuroraB kinase (mouse monoclonal) | BD Transduction Laboratories | #611082, RRID:AB_2227708 | 1:200 |
| Antibody | Anti-Ki67 (Rabbit monoclonal) | Cell Marque | #275R | 1:200 |
| Antibody | Anti-MCT4 (rabbit polyclonal) | Santa Cruz | #SC50329, RRID:AB_2189333 | 1:200 |
| Antibody | Anti-PCNA (mouse monoclonal) | DAKO | #M0879, RRID:AB_2160651 | 1:800 |
| Antibody | Anti-GFP (chicken polyclonal) | Aves | #GFP-1010, RRID:AB_2307313 | 1:1000 |
| Antibody | Anti-Mef2C (rabbit polyclonal) | Santa Cruz/Biorbyt | #SC313, RRID:AB_631920 / #orb256682 | Both 1:1000 |
| Chemical compound, drug | 2-Deoxyglucose | Sigma-Aldrich | #D6134 | 1 mg/g or 3 mM |
| Chemical compound, drug | Lonidamine | Sigma-Aldrich | L4900 | 80 uM |
| Chemical compound, drug | 2NBDG | Caymanchem | #11046 | 400 uM |
| Chemical compound, drug | AG1478 | Selleck Chemical | S2728 | 5M, from 10 mM stock in DMSO |
| Chemical compound, drug | Doxycycline | Harlan Laboratories | TD02503 | |
| Chemical compound, drug | Trizol | Life technologies | #15596026 | |
| Chemical compound, drug | Fast SYBR Green Master Mix | Applied Biosystems | #4385612 | |
| Chemical compound, drug | Collagenase type II | Gibco | 17101015 | 0.1% |
| Chemical compound, drug | TrypLE Express Enzyme (1x), phenol red | Gibco | 12605036 | |
| Software, algorithm | FIJI | Schindelin et al., 2012 | Version 2.0.0, RRID:SCR_002285 | |
| Software, algorithm | RaceID2/StemID | Grün, D. et al. De Novo Prediction of Stem Cell Identity using Single-Cell Transcriptome Data. Cell Stem Cell 19, 266–277 (2016). | RRID:SCR_017242 | |
| Software, algorithm | Rstudio | Rstudio | RRID:SCR_000432 | Version 1.2.1335 |
| Software, algorithm | Imaris | Bitplane | RRID:SCR_0007370 | V9.3.1 |
| Software, algorithm | Gene Set Enrichment Analysis | Genepattern, Broad Institute | RRID:SCR_003199 | # of permutations = 1000 |
| Commercial assay, kit | Superscript III First strand synthesis system | Thermo Fisher Scientific | #18080051 | Input 200 ng RNA |
| Commercial assay, kit | MiRNeasy | Qiagen | 217004 | |
| Commercial assay, kit | High capacity cDNA Reverse Transcription kit | Applied Biosystems | 4374966 | Input 1 ug RNA |
| Commercial assay, kit | Neonatal Dissociation kit | Miltenyi Biotec | 130-098-373 | |
| Commercial assay, kit | TruSeq small RNA primers | Illumina | 20005613 |