(A) Expression of Dectin-1 on WT cDC2s. Numbers indicate mean fluorescence intensity (GeoMFI). (B) IL-17A levels produced by OT-II cells co-cultured with WT splenic cDC2s treated with or without curdlan (10 μg/ml). (C) qPCR of lineage commitment factors in OT-II cells co-cultured with curdlan-treated and untreated WT cDC2s. qPCR of (D) TFs and (E) IL-23 in the WT cDC2s treated with curdlan for the indicated time points. Two-way ANOVA; n = 3 in each group. (F) IL-17A levels produced by OT-II cells co-cultured with WT BM-APC pre-treated with Rp-cAMP (50 μM), or 666–15 (1 μM) 16 hr prior to curdlan treatment. (G) qPCR of Irf4 in WT cDC2s treated with or without curdlan in the presence of inhibitors of CREB (666–15, 1 μM) or PKA (Rp-cAMP, 50 μM). Two-way ANOVA; n = 3 in each group. Data are representative of three independent experiments; *p<0.05, **p<0.01, ***p<0.001.