Skip to main content
. 2020 Feb 4;9:e49416. doi: 10.7554/eLife.49416

Figure 3. Stimulation of DCs via Dectin-1 (a PPR) regulates IRF4 and KLF4 expression, and induce Th17 differentiation.

(A) Expression of Dectin-1 on WT cDC2s. Numbers indicate mean fluorescence intensity (GeoMFI). (B) IL-17A levels produced by OT-II cells co-cultured with WT splenic cDC2s treated with or without curdlan (10 μg/ml). (C) qPCR of lineage commitment factors in OT-II cells co-cultured with curdlan-treated and untreated WT cDC2s. qPCR of (D) TFs and (E) IL-23 in the WT cDC2s treated with curdlan for the indicated time points. Two-way ANOVA; n = 3 in each group. (F) IL-17A levels produced by OT-II cells co-cultured with WT BM-APC pre-treated with Rp-cAMP (50 μM), or 666–15 (1 μM) 16 hr prior to curdlan treatment. (G) qPCR of Irf4 in WT cDC2s treated with or without curdlan in the presence of inhibitors of CREB (666–15, 1 μM) or PKA (Rp-cAMP, 50 μM). Two-way ANOVA; n = 3 in each group. Data are representative of three independent experiments; *p<0.05, **p<0.01, ***p<0.001.

Figure 3.

Figure 3—figure supplement 1. Curdlan does not affect the expression of Irf4 and subsequent T cell differentiation in cDC1s.

Figure 3—figure supplement 1.

(A) Expression of Dectin-1 on WT cDC1s. Numbers indicate mean fluorescence intensity (GeoMFI). (B) IL-17A and (C) IFN-γ levels from anti-CD3/28 Ab-stimulated OT-II cells co-cultured with cDC1s from WT mice pretreated with or without curdlan. Relative expression of (D) Irf4 and (E) Il23a in WT cDC1s treated with curdlan for the indicated time points. Data are representative of three independent experiments.