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. 2020 Feb 4;9:e49416. doi: 10.7554/eLife.49416

Author response image 5. FOXP3 expression in T1/ST2+ cells.

Author response image 5.

OT-II CD4+ T cells were co-cultured with GnasΔCD11c BM-APCs to generate memory Th2 cells (first co-culture). From the first co-culture, T1/ST2+ cells were FACS sorted and then used for co-culture with WT cDC2s pretreated with or without CPT or Cholera toxin (CT) (second co-culture). (A) Memory T cell marker and FOXP3 were analyzed in the first co-cultured T cells. (B) FOXP3 were analyzed in the second co-cultured T cells.