Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6J (B6) | The Jackson Laboratory | RRID:IMSR_JAX:000664 | |
| Strain, strain background (Mus musculus, B6) | CD11c-Cre | The Jackson Laboratory | RRID:IMSR_JAX:008068 | |
| Strain, strain background (Mus musculus, B6) | OT-II | The Jackson Laboratory | RRID:IMSR_JAX:004194) | |
| Strain, strain background (Mus musculus, B6) | IL17A-eGFP | The Jackson Laboratory | RRID:IMSR_JAX:018472 | |
| Strain, strain background (Mus musculus, B6) | Irf4fl/fl | The Jackson Laboratory | RRID:IMSR_JAX:009380 | |
| Strain, strain background (Mus musculus, B6) | Gnas fl/fl | Lee Weinstein (NIH) | MGI:3609165 PMID: 15883378 |
|
| Strain, strain background (Mus musculus, B6) | 4Get | Dr. M. Kronenberg (LAI, San Diego, CA) | RRID:IMSR_JAX:004190 | |
| Strain, strain background (Mus musculus, B6) | Irf4-inducible (Irf4-/-) (Irf4i) | Dr. R. Sciammas (University of California, Davis) | PMID: 23684984 | |
| Strain, strain background (Mus musculus, B6) | Irf5-/- | Dr. I. R. Rifkin (Boston University) | PMID: 25595782 | |
| Strain, strain background (Mus musculus, B6) | Il17a-/- | Dr. Y. Iwakura (The University of Tokyo, Japan) | MGI:2388010 PMID: 12354389 |
|
| Cell line (Homo-sapiens) | MUTZ-3 | Dr. Martin L. Yarmush (Rutgers University, New Jersey) | PMID: 29682279 | |
| Cell line (Homo-sapiens) | THP-1 | ATCC | RRID:CVCL_0006 | |
| Cell line (Homo-sapiens) | HL-60 | ATCC | RRID:CVCL_0002 | |
| Antibody | PE anti-mouse monoclonal CD11c | eBioscience | Cat# 12-0114-82 | FACS (1:200) |
| Antibody | FITC anti-mouse monoclonal CD11c | BioLegend | Cat# 117306 | FACS (1:200) |
| Antibody | APC anti-mouse monoclonal CD8α | BD Bioscience | Cat# 553035 | FACS (1:200) |
| Antibody | FITC anti-mouse monoclonal CD8α | BD Bioscience | Cat# 553031 | FACS (1:200) |
| Antibody | APC anti-mouse monoclonal CD11b | eBioscience | Cat# 17-0112-82 | FACS (1:200) |
| Antibody | PE/Cy7 anti-mouse monoclonal CD11b | BioLegend | Cat# 101216 | FACS (1:100) |
| Antibody | PerCP-eFluor 710 anti-mouse monoclonal CD135 | eBioscience | Cat#46-1351-80 | FACS (1:200) |
| Antibody | PerCP-eFluor710 anti-human/mouse monoclonal IRF4 | eBioscience | Cat# 46-9858-80 | FACS (1:200) |
| Antibody | PE anti-human/mouse monoclonal IRF8 | R and D Systems | Cat# IC8447P | FACS (1:200) |
| Antibody | PE anti-mouse monoclonal IRF5 | Invitrogen | Cat# PCS-201–012 | FACS (1:200) |
| Antibody | PerCP anti-mouse monoclonal CD4 | BD Bioscience | Cat# 553052 | FACS (1:200) |
| Antibody | FITC anti-mouse monoclonal CD4 | BD Bioscience | Cat# 553729 | FACS (1:200) |
| Antibody | PE anti-mouse monoclonal IL-33Rα | BioLegend | Cat# 145303 | FACS (1:200) |
| Antibody | PEcy7 anti-mouse monoclonal CD44 | eBioscience | Cat# 25-0441-82 | FACS (1:200) |
| Antibody | APC anti-mouse monoclonal CD62L | eBioscience | Cat# 17-0621-83 | FACS (1:200) |
| Antibody | APC anti-mouse monoclonal FOXP3 | eBioscience | Cat# 17-5773-82 | FACS (1:200) |
| Antibody | PE anti-mouse monoclonal Dectin-1 | BioLegend | Cat# 144303 | FACS (1:200) |
| Antibody | anti-mouse monoclonal CD3e (2C11) | BioXcell | Cat# BE0001-1FAB | 10 μg/ml |
| Antibody | anti-mouse monoclonal CD28 | BioXcell | Cat# BE0015-5 | 1 μg/ml |
| Commercial assay or kit | EasySep Mouse Naïve CD4+ T Cell Isolation Kit | StemCell Technologies | Cat# 19765 | |
| Peptide, recombinant protein | Recombinant Mouse GM-CSF | BioLegend | Cat# 576302 | 10 ng/ml |
| Peptide, recombinant protein | Recombinant Human GM-CSF | BioLegend | Cat# 572903 | 100 ng/ml |
| Peptide, recombinant protein | TGF-β | R and D systems | Cat# 240-B-002 | 10 ng/ml |
| Peptide, recombinant protein | TNF-α | R and D systems | Cat# 210-TA-005 | 2.5 ng/ml |
| Peptide, recombinant protein | rhIL-4 | BioLegend | Cat# 574002 | 100 ng/ml |
| Chemical compound, drug | Calcium ionophore A23187 | Sigma-Aldrich | Cat# C7522 | 180 ng/ml |
| Chemical compound, drug | CPT-cAMP | Sigma-Aldrich | Cat# C3912 | 50 μM |
| Chemical compound, drug | Prostaglandin E2 | Sigma-Aldrich | Cat# P5640 | 10 μM |
| Chemical compound, drug | Forskolin | Sigma-Aldrich | Cat# F6886 | 10 μM |
| Chemical compound, drug | Pertussis toxin | Sigma-Aldrich | Cat# P7208 | 100 ng/ml |
| Chemical compound, drug | Phorbol 12-myristate 13-acetate | Sigma-Aldrich | Cat# P8139 | 50 ng/ml |
| Chemical compound, drug | Ionomycin | Sigma-Aldrich | Cat# I3909 | 1 μM |
| Chemical compound, drug | Doxycycline | Sigma-Aldrich | Cat# D9891 | 200 ng/ml |
| Chemical compound, drug | CREB inhibitor (666-15) | Millipore Sigma | Cat# 5383410001 | 1 μM |
| Chemical compound, drug | Cholera toxin | List biological laboratories | Cat# 101B | 1 μg/ml |
| Chemical compound, drug | Ovalbumin | Worthington Biochemical | Cat# LS003054 | 100 μg/ml |
| Chemical compound, drug | MHC class II OVA peptide | GenScript | Cat# RP10610 | 1 μg/ml |
| Chemical compound, drug | HDM extract | Greer Laboratories | Cat# XPB82D3A2.5 | |
| Chemical compound, drug | Rp-cAMP | BioLog | Cat# A002S | 50 μM |
| Chemical compound, drug | Rolipram | Tocris Bioscience | Cat# 0905 | 10 μM |
| Chemical compound, drug | CE3F4 | Tocris Bioscience | Cat# 4793 | 10 μM |
| Chemical compound, drug | Curdlan | Wako Chemicals | Cat# 034–09901 | 10 μg/ml |