Cell line	RRID	Growth medium (FBS: heat-inactivated fetal bovine serum; P/S: penicillin/streptomycin)	Temp	CO2
184-B5	CVCL_4688	MEBM (Lonza/Clonetics; MEGM Kit #CC-3150) + 1ng/ml cholera toxin (unfiltered)	37°C	5%
AU-565	CVCL_1074	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
BT-20	CVCL_0178	EMEM + 10% FBS + 1% P/S	37°C	5%
BT-474	CVCL_0179	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
BT-483	CVCL_2319	RPMI-1640 + 20% FBS + 1% P/S + 0.01mg/ml bovine insulin	37°C	5%
BT-549	CVCL_1092	RPMI-1640 + 10% FBS + 1% P/S + 0.023IU/ml insulin	37°C	5%
CAL-120	CVCL_1104	DMEM + 10% FBS + 1% P/S	37°C	5%
CAL-51	CVCL_1110	DMEM + 20% FBS + 1% P/S	37°C	5%
CAL-85-1	CVCL_1114	DMEM + 10% FBS + 1% P/S	37°C	5%
CAMA-1	CVCL_1115	EMEM + 10% FBS + 1% P/S	37°C	5%
HCC1143	CVCL_1245	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
HCC1395	CVCL_1249	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
HCC1419	CVCL_1251	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
HCC1428	CVCL_1252	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
HCC1500	CVCL_1254	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
HCC1569	CVCL_1255	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
HCC1806	CVCL_1258	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
HCC1937	CVCL_0290	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
HCC1954	CVCL_1259	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
HCC202	CVCL_2062	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
HCC38	CVCL_1267	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
HCC70	CVCL_1270	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
Hs 578T	CVCL_0332	DMEM + 10% FBS + 1% P/S	37°C	5%
hTERT-HME1	CVCL_3383	MEBM + Lonza CC-3150 kit	37°C	5%
MCF-10A	CVCL_0598	1:1 DMEM and Ham’s F12 + 20ng/ml human epidermal growth factor + 100ng/ml cholera toxin + 0.01mg/ml bovine insulin + 500ng/ml hydrocortisone, 95% + 5% horse serum	37°C	5%
MCF-10F	CVCL_3633	1:1 DMEM and Ham’s F12 + 20ng/ml human epidermal growth factor + 100ng/ml cholera toxin + 0.01mg/ml bovine insulin + 500ng/ml hydrocortisone, 95% + 5% horse serum	37°C	5%
MCF-12A	CVCL_3744	1:1 DMEM and Ham’s F12 + 20ng/ml human epidermal growth factor + 100ng/ml cholera toxin + 0.01mg/ml bovine insulin + 500ng/ml hydrocortisone, 95% + 5% horse serum	37°C	5%
MCF-7	CVCL_0031	DMEM + 10% FBS + 1% P/S	37°C	5%
MDA-MB-134-VI	CVCL_0617	Leibovitz’s L-15 + 20% FBS (not heat-inactivated) + 1% P/S	37°C	0%
MDA-MB-157	CVCL_0618	Leibovitz’s L-15 +10% FBS (not heat-inactivated) + 1% P/S	37°C	0%
MDA-MB-175-VII	CVCL_1400	Leibovitz’s L-15 +10% FBS (not heat-inactivated) + 1% P/S	37°C	0%
MDA-MB-231	CVCL_0062	DMEM (or L-15) + 10% FBS (not heat-inactivated) + 1% P/S	37°C	0%
MDA-MB-361	CVCL_0620	Leibovitz’s L-15 + 20% FBS (not heat-inactivated) + 1% P/S	37°C	0%
MDA-MB-415	CVCL_0621	Leibovitz’s L-15 + 15% FBS (not heat-inactivated) + 1% P/S + 2mM L-glutamine + 10mg/l insulin	37°C	0%
MDA-MB-436	CVCL_0623	Leibovitz’s L-15 + 10% FBS (not heat-inactivated) + 1% P/S + 10mg/l insulin	37°C	0%
MDA-MB-453	CVCL_0418	Leibovitz’s L-15 + 10% FBS (not heat-inactivated) + 1% P/S	37°C	0%
MDA-MB-468	CVCL_0419	Leibovitz’s L-15 + 10% FBS (not heat-inactivated) + 1% P/S	37°C	0%
SK-BR-3	CVCL_0033	McCoy’s 5a + 10% FBS + 1% P/S	37°C	0%
SUM102PT	CVCL_3421	Ham’s F-12 + 1% P/S + 5 μg/ml insulin + 1ug/ml hydrocortisone + 10ng/mL epidermal growth factor + 5mM ethanolamine + 10mM HEPES + 5ug/mL transferrin + 10nM T3 + 50nM sodium selenite + 1g/L BSA	37°C	5%
SUM1315MO2	CVCL_5589	Ham’s F-12 + 5% FBS + 1% P/S + 10ng/ml human epidermal growth factor + 5μg/ml insulin + 10 mM HEPES	37°C	5%
SUM149PT	CVCL_3422	Ham’s F-12 + 5% FBS + 1% P/S + 1ug/ml hydrocortisone + 5μg/ml insulin + 10mM HEPES	37°C	5%
SUM159PT	CVCL_5423	Ham’s F-12 + 5% FBS + 1% P/S + 1ng/ml hydrocortisone + 5μg/ml insulin + 10mM HEPES	37°C	5%
SUM225CWN	CVCL_5593	Ham’s F-12 + 5% FBS + 1% P/S + 5μg/ml insulin + 1ug/ml hydrocortisone + 10mM HEPES	37°C	5%
SUM44PE	CVCL_3424	Ham’s F-12 + 1% P/S + 5μg/ml insulin + 1ug/ml hydrocortisone + 5mM ethanolamine + 10mM HEPES + 5ug/mL transferrin + 10nM T3 + 50nM sodium selenite + 1g/L BSA	37°C	5%
SUM52PE	CVCL_3425	Ham’s F-12 + 5% FBS + 1% P/S + 5 μg/ml insulin + 1ug/ml hydrocortisone + 10mM HEPES	37°C	5%
T-47D	CVCL_0553	RPMI-1640 + 10% FBS + 1% P/S + 0.2units/ml bovine insulin	37°C	5%
UACC-812	CVCL_1781	Leibovitz’s L-15 + 20% FBS (not heat-inactivated) + 1% P/S + 2mM L-glutamine + 20ng/ml human EGF	37°C	0%
UACC-893	CVCL_1782	Leibovitz’s L-15 + 10% FBS (not heat-inactivated) + 1% P/S	37°C	0%
ZR-75-1	CVCL_0588	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%
ZR-75-30	CVCL_1661	RPMI-1640 + 10% FBS + 1% P/S	37°C	5%