FIGURE 1.

RELA interactome. Upstream regulator analysis of IPA identified RELA as one of the most activated transcription factors (activation z score = 5.58). Here we show selected interactome of RELA which has significant overlap (P value: 1.6E−11) with our gene set (GSE105088) of IL-1-induced genes in LNCaP. A complete list of proteins in this RELA interactome can be found in Table S1. Red color indicates the gene encoding the protein was significantly upregulated (IL-1-induced) in our gene set and green color indicates downregulation (IL-1-inhibited). Orange color indicates predicted activation consistent with knowledge base and blue color indicates predicted inhibition, yellow color indicated inconsistent findings between our data and the knowledge base. Solid arrow indicates direct relationship and dotted line indicates indirect relationship. IPA knowledge base predicts that RELA activates AR expression in IL-1-treated cells which is inconsistent (yellow arrow) with our gene expression data (GSE105088). IL, interleukin; IPA, ingenuity pathway analysis [Color figure can be viewed at wileyonlinelibrary.com]