Fig. 6.

MSCs transplantation reversed β cell dedifferentiation and improved β cell function of db/db mice. (A) Study design of the MSCs transplantation in db/db mice. (B) Immunofluorescence with Insulin (Ins, green), Foxo1 (red) and DAPI (blue) in db/db mice islets of the MSCs treatment group and the control group 4 weeks after treatment. Scale bars=20 μm. (C) Quantification of the percentage of Foxo1⁻Ins⁺ and Foxo1⁺Ins⁺ cells per islet (n = 5 per group). (D) Immunofluorescence with Insulin (Ins, green), Aldh1a3 (red) and DAPI (blue) of db/db mouse islets with MSCs treatment group and control group 4 weeks after treatment. Scale bars=20 μm. (E) Quantification of the percentage of Aldh1a3⁺Ins⁺ and Aldh1a3⁻Ins⁺cells per islet (n = 5 per group). (F) Weight of the db/db mice of the MSCs treatment group and control group (n = 8 per group). (G) Daily food intake of the db/db mice of the MSCs treatment group and control group (n = 8 per group). (H) Non-fasting glucose measurement of db/db mice in the MSCs treatment group and control group (n = 8 per group). (I) OGTT of the db/db mice of the MSCs treatment group and control group 4 weeks after the treatment. (J) AUC of the OGTT curve of the db/db mice of the MSCs treatment group and control group 4 weeks after the treatment (n = 6–8 per group). Data were shown as mean±SEM. *p<0.05, **p<0.01, ***p<0.001.