Fig. 6.

Dioscin suppresses xenograft tumor growth in vivo. (a–c) HT29 cells were injected into nude mice and treated with vehicle control or dioscin when tumor volume reached 100 mm³. Tumor volume was measured by caliper (a), tumor mass was photographed (b), and weight (c). ***p < 0.001. (d–f) SW620 cells were injected into nude mice and treated with vehicle control or dioscin when tumor volume reached 100 mm³. Tumor volume was measured by caliper (d), tumor mass was photographed (e), and weight (f). ***p < 0.001. (g) Immunohistochemistry (IHC) staining analysis of Ki67, Skp2, and HK2 in HT29 xenograft tumors. ***p < 0.001. (h) WCE from HT29 xenograft tumors were subjected to in vivo ubiquitination analysis. Scale bar, 25 μm.