Fig. 1.

PHF8 expression is elevated in HER2+ breast cancers and is upregulated by HER2.a. Immunohistochemistry (IHC) was used to measure PHF8 levels in breast cancer tissue arrays. Representative PHF8 staining of breast cancer and normal adjacent tissues: Basal like, Luminal A, Luminal B, and HER2+ samples. Magnification: 200 ×, bar = 10 μm. b. Western blot and RT-PCR analyses of the levels of PHF8 (upper panel) and its mRNA (lower panel) in MCF10A and MCF7 cells with or without overexpression of HER2. c. Western blotting and RT-PCR analyses of PHF8 protein (upper panel) and mRNA (lower panel) levels in MCF10A-HER2 and MCF7-HER2 cells with or without HER2 knockdown. * p ≤ 0.05; ** p ≤ 0.01.