Fig. 5.

Functional impact of PHF8 on HER2-induced tumourigeneses and inflammatory response in vivo. a.Schematic of floxed Phf8 and the corresponding amino acid sequence. b. Genotyping of Her2, flox, and Cre using tail DNA from the indicated mouse. kbp: Kilobase pair. c. Western blotting analysis of PHF8 and HER2 levels of mice with the indicated genotypes. d–f. Tumour onset date (d), tumour weight (e), and tumour ratio (f) of WT to Phf8 KO mice. Each point represents an individual animal. The Student t-test was used to calculate the significance of differences between the KO and WT groups (n = 86). g. IHC analysis of PHF8, Ki67, CD4, and CD8 levels in tumour tissues from Phf8 KO and WT mice (magnification, 200 ×, bar = 10 μm. h. Comparison of infiltrating T cells in tumours from WT and Phf8 KO mice. The percentages of intraepithelial and stromal tumour-infiltrating CD8+ and CD4+ T cells were quantified in 200x fields. I. Western blotting analysis of HER2 and PHF8 levels in primary tumour cell lines from Phf8 KO and WT mice. J. RT-qPCR mRNA levels of Il-6 in primary tumour cell lines. Il-6 expression was normalised by both Rpl13a and β-actin. * p ≤ 0.05.