Table 1.
Kinetics of endogenous and heterologous expressed xylose transporters in S. cerevisiae.
| D-xylose | D-glucose | ||||
|---|---|---|---|---|---|
| Km (mM) | Vmax (nmol/mgDW.min) | Km (mM) | Vmax (nmol/mgDW.min) | Author | |
| Hxt36 | 107.9 ± 12.1 | 62.5 ± 5.9 | 6.1 ± 0.1 | 60.2 ± 2 | Nijland et al., 2014 |
| Hxt36 N367I | 39.8 ± 5.6 | 23 ± 3 | – | ±0 | Nijland et al., 2014 |
| Hxt36 N367A | 24.9 ± 3.4 | 29.1 ± 0.4 | 170.7 ± 37.8 | 70.7 ± 8.4 | Nijland et al., 2014 |
| Hxt7 | 130 ± 9 | 110 ± 7 | nda | nda | Saloheimo et al., 2007 |
| Hxt7 | 200.3 ± 13.2 | 67 ± 2 | 0.5 ± 0.1 | 26 ± 1.1 | Farwick et al., 2014 |
| Hxt7 N370S | 169.9 ± 26.3 | 24.1 ± 1.6 | – | ±0 | Farwick et al., 2014 |
| Hxt7 | 161.2 ± 22 | 101.6 ± 6.5 | nda | nda | Reider Apel et al., 2016 |
| Hxt7 F79S | 228.8 ± 45.9 | 186.4 ± 20.1 | nda | nda | Reider Apel et al., 2016 |
| Gal2 | 225.6 ± 15.8 | 91.3 ± 3.2 | 1.5 ± 0.2 | 27.2 ± 0.9 | Farwick et al., 2014 |
| Gal2 N376F | 91.4 ± 8.9 | 37.3 ± 1.3 | – | ±0 | Farwick et al., 2014 |
| Gal2 N376V | 168.3 ± 31.6 | 28.4 ± 2.3 | 22.1 ± 1.8 | 50.5 ± 1.4 | Farwick et al., 2014 |
| Hxt11 | 84.2 ± 10 | 84.6 ± 2.4 | 33.4 ± 2.1 | 156.4 ± 7.6 | Shin et al., 2015 |
| Hxt11 N376D | 106.7 ± 21.7 | 86.5 ± 2 | 87 ± 6.4 | 197.8 ± 11.4 | Shin et al., 2015 |
| Hxt11 N376T | 46.7 ± 2.7 | 76.2 ± 4.8 | 194.4 ± 47.9 | 238.6 ± 7.4 | Shin et al., 2015 |
| Hxt11 N376M | 50.1 ± 9.7 | 65 ± 6.8 | 144.9 ± 36 | 143 ± 17.2 | Shin et al., 2015 |
| CiGxf1 | 48.7 ± 6.5 | 10.8 ± 1.0b | 2 ± 0.6 | 1.4 ± 0.2b | Leandro et al., 2006 |
| CiGxs1 | 0.4 ± 0.1 | 0.39 ± 0.09 | 0.012 ± 0.004 | 0.0043 ± 0.0003 | Leandro et al., 2006 |
| CiGxs1 | 0.026 ± 0.06 | 7.23 ± 0.6 | nda | nda | Young et al., 2014 |
| CiGxs1 F38I39M40 | 0.72 ± 0.12 | 15.01 ± 2.38 | ±0 | ±0 | Young et al., 2014 |
| CiGxs1 | 0.08 ± 0.02 | 5.68 ± 0.3 | nda | nda | Young et al., 2012 |
| CiGxs1 2.1 | 1.58 ± 0.49 | 11.03 ± 3.71 | nda | nda | Young et al., 2012 |
| CiGxs1 2.2 | 1.2 ± 0.05 | 3.52 ± 0.27 | nda | nda | Young et al., 2012 |
| CiGxs1 2.3 | 1.25 ± 0.32 | 10.91 ± 1.44 | nda | nda | Young et al., 2012 |
| PsSut1 | 145 ± 1 | 132 ± 1 | 1.5 ± 0.1 | 45 ± 1 | Weierstall et al., 1999 |
| PsSut2 | 49 ± 1 | 41 ± 1 | 1.1 ± 0.1/55 ± 11c | 3.3 ± 0.1/28 ± 4c | Weierstall et al., 1999 |
| PsSut3 | 103 ± 3 | 87 ± 2 | 0.8 ± 0.1/31 ± 0.1c | 3.7 ± 0.1/22 ± 0.1c | Weierstall et al., 1999 |
| SsXut3 | 4.09 ± 1.08 | 11.31 ± 2.31 | nda | nda | Young et al., 2012 |
| SsXut3 1.1 | 2.02 ± 0.40 | 15.67 ± 0.87 | nda | nda | Young et al., 2012 |
| SsXut3 1.2 | 1.73 ± 0.93 | 6.65 ± 2.64 | nda | nda | Young et al., 2012 |
| AnHxtB | 0.54 ± 0.08 | 19 ± 1.33 | nda | nda | dos Reis et al., 2016 |
a
Not determined.
b
Data extracted from figure.
c
Data fitted more accurately to two transport components.