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. 2020 Jan 29;7:464. doi: 10.3389/fbioe.2019.00464

Table 2.

Kinetics of endogenous and heterologous expressed L-arabinose transporters in S. cerevisiae.

	L-arabinose		D-xylose		D-glucose
	K_m (mM)	V_textsfmax (nmol/min.mgDW)	K_textsfm (mM)	V_textsfmax (nmol/min.mgDW)	K_textsfm (mM)	V_textsfmax (nmol/min.mgDW)	Author
Gal2	371 ± 19	341 ± 7	nd^a	nd^a	nd^a	nd^a	Knoshaug et al., 2015
Gal2	57 ± 11	2.2 ± 0.26	nd^a	nd^a	nd^a	nd^a	Subtil and Boles, 2011
Gal2	nd^a	nd^a	225 ± 16	91 ± 3.2	1.5 ± 0.2	27.2 ± 0.9	Farwick et al., 2014
Gal2	335 ± 21	75 ± 5	nd^a	nd^a	1.9 ± 21	26 ± 1	Verhoeven et al., 2018
Gal2 N376I	117 ± 16	39 ± 3	nd^a	nd^a	101 ± 47	32 ± 18	Verhoeven et al., 2018
Gal2 N376S	186 ± 33	64 ± 2	nd^a	nd^a	38 ± 1	28 ± 1	Verhoeven et al., 2018
Gal2 N376T	171 ± 17	65 ± 2	nd^a	nd^a	57 ± 1	17 ± 4	Verhoeven et al., 2018
Gal2 N376I T89I	103 ± 40	30 ± 2	nd^a	nd^a	±0	±0	Verhoeven et al., 2018
AmLat1	nd^a	0.2 ± 0.015^b	nd^a	nd^a	No growth^c	No growth^c	Verho et al., 2011
AmLat1-mCh	0.03	3 x > amLat1	nd^a	nd^a	nd^a	nd^a	Londesborough et al., 2014
AmLat2	nd^a	4 ± 0.25^d	nd^a	nd^a	No growth^c	No growth^c	Verho et al., 2011
KmAxt1	263 ± 57	57 ± 6	27 ± 3	3.8 ± 0.02	No growth^c, GI^d	No growth^c, GI^d	Knoshaug et al., 2015
PgAxt1	0.13 ± 0.04	18 ± 0.8	65 ± 8	8.7 ± 0.3	no growth^c, GI^d	No growth^c, GI^d	Knoshaug et al., 2015
SsAraT	3.8 ± 1.7	0.4 ± 1.7	nd^a	nd^a	nd^a	nd^a	Subtil and Boles, 2011
AtStp2	4.5 ± 2.2	0.6 ± 0.08	nd^a	nd^a	nd^a	nd^a	Subtil and Boles, 2011
PcAraT	0.13 ± 0.03	5.3 ± 0.2	±0	±0	±0	±0	Bracher et al., 2018
NcLat1	58 ± 4	1945 ± 50	nd^a	nd^a	GI^d	GI^d	Li et al., 2015
MtLat1	29 ± 4	172 ± 6	nd^a	nd^a	GI^d	GI^d	Li et al., 2015

^a

Not determined.

^b

Uptake at 100 mM L-arabinose.

^c

No growth observed on D-glucose.

^d

L-arabinose uptake inhibited by D-glucose (GI; D-glucose inhibited).