Table 3. Conventional methods for detection of A(H1N1) virus.

Serial No	Detection method	Sample type for detection	Detection time	Gene/protein	Sensitivity/L.O.D	References
1	Cell culture	H1N1 Virus	7days	-	86-94%	[33]
2	ELISA	H1N1 Virus	4-5 hours	HA antibody	93.7%	[52]
3	RIDT kits	H1N1 Virus	< 30min	NA/antibody	1.13 HAU	[62]
4	RT-LAMP	Extracted RNA	3-4 hours	HA gene	93.8%	[70]
5	Real-Time PCR	Extracted RNA	3-4 hour	HA gene	5copies/reaction	[107]
6	HI assay	Sera	-	HA	92%	[110]
7	Conventional RT-PCR	Extracted RNA	3-4 hour	HA gene	1.0104 TCID50	[111]

RIDT= Rapid influenza detection test, H1=Hemagglutinin 1, RT-LAMP= Reverse transcription-Loop mediated isothermal amplification, HA= Hemagglutinin, NA= Neuraminidase, TCID= Tissue Culture Infective Dose