The BV2 cells were treated with LPS (1 µg/ml), and transfected with Ctrl-siRNA, si-PTX3, si-PTX3+pcDNA3.1 and si-PTX3+pcDNA3.1-TLR4. (A) The level of IL-6 in each group. (B,A) The level of NO in each group. (C) The level of TNF-α in each group. (D) The protein expression of COX-2 in each group. (E) The protein expression of iNOS in each group. ‘*’ means compared with the untreated group at P<0.05, and ‘#’ means compared with the si-PTX3+pcDNA3.1 group at P<0.05. GAPDH was used as an invariant internal control for calculating protein fold changes.