Figure 4.

The expression analysis of VbWRKY32 in leaves of Verbena bonariensis. (A) Relative expression level of VbWRKY32 in roots, stems, and leaves of WT lines at 28°C. (B) Cold treatment. Control stands for non-stress treatment and at 28°C. The process of temperature treatment was continuous. The temperature was at 28°C, then drop to 4°C for 12 h, 24 h, and then to −4°C for 1, 2, 4, 5 h. Data indicate means ± standard errors (SE) of three biological replicates. The different letters above the columns represent significant differences (P < 0.05) on the basis of Duncan’s multiple range test.