Figure 1.

Sample collection and analysis. Fecal samples were collected from three young and healthy Korean adults. Each fecal sample was divided into two parts. For non-stabilized (NS) samples, the fecal sample was aliquoted into 24 cryotubes. For samples preserved with OMNIgene-GUT solution (OMNI samples), each fecal sample was mixed with OMNIgene-GUT solution using four OMNIgene-GUT tubes, pooled into one Falcon tube, and then aliquoted into 24 cryotubes. Samples were stored at room temperature until they were relocated to a deep freezer at four time points. The microbiome and metabolite profiles were analyzed for each specimen.